Technology Evolving Solutions

Designing Smart Technology and Automation to Solve Global Problems

TES's Mission

Stop the spread of Huanglongbing(HLB) and other citrus diseases, work to eliminate them wherever they are established, and in the process, train the next generation of biologist, scientist, and engineers

C.H.A.B.S Testing

C

Citrus tissue type needs to be evaluated in the development of CHABS testing protocols (leaves, budwood, feather-flush/nymphs, roots, and psyllids). For example, leaves may be the best tissue type for bulk screening, whereas roots may have a higher HLB titer than leaves, providing more sensitivity in the detection of HLB in infected areas.

H

Hierarchal testing begins with the most cost-effective method for testing a large number of trees/leaves (bulk) and concludes with a more sensitive test of individual trees in an area of concern. After discovering the area where HLB is located, a more thorough test is then utilized to attempt to ensure that all infected trees are found.

A

Automation is critical in developing a cost effective and consistent process. Eliminating the current labor intensive hand chopping was essential in evolving bulk processing and was accomplished with the instruments shown under automation. Larger leaf samples were prohibitive with conventional methods, due to workers acquiring repetitive injuries.

B

Bulk testing is currently the most cost-effective way to screen an orchard for the presence of HLB. Bulk testing, combined with automation, not only significantly lowers labor costs by removing hand chopping of a large volume of leaves, but also significantly lowers material costs.

S

System approach to HLB testing. Evaluate each step in the process, which includes developing bulk processing protocols, automation of citrus tissue processing through instrument development, evolving of a paperless sample tracking system, citrus sample collection, and a host of other issues

Products

Budwood Tissue Extractor
BTE

The BTE is an attachment for our Tissue Extractor(TE) system that processes budwood. This replaces the hand chopping, freeze drying, and bead beating of the current regulatory processes. On top of the systems and equipment it replaces, it is compatible with TES's automatic sample tracking system. The median processing time of the BTE over ~1000 samples is 02:28 compared to 08:34 average of the CCTEA.

Click To See BTE Demonstration Video

Leaf Tissue Extractor
LTE

The LTE is an attachment for our Tissue Extractor(TE) system that processes leaves. This replaces the hand chopping, freeze drying, and bead beating of the current regulatory processes. On top of the systems and equipment it replaces, it is compatible with TES's automatic sample tracking system. The median processing time of the LTE over ~600 samples is 04:38 min compared to 14:00 min average of the CCDFA.

Click To See LTE Demonstration Video

Bulk Leaf Tissue Extractor
BLTE

The BLTE is an attachment for our Tissue Extractor(TE) system that processes leaves and root. The BLTE is designed to process large quantities of plant tissue. Combined with our bulk extraction process, it can test much larger quantities of material than the current regulatory standard with little to no cq shift

Preliminary BLTE data at CDFA

Sample	CDFA cq1	CDFA cq2
1 HLB + 11 healthy leaves	27.05	27.46
1 HLB + 23 healthy leaves	26.02	26.84
1 HLB + 47 healthy leaves	26.70	26.72

Paperless Automated Sample Tracking System
PASTS

The PAST system is a paperless automatic sample tracking system that tracks samples from when they were collected to when they have PCR results. Our system automatically records timestamps, sample weights, and equipment information to provide the user with everything they could want about a sample. This tracking system leverages NFC technology to link trees with samples or transfer information to corresponding systems.

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